Color Alteration and Growth Performance of Spiny Lobster (Panulirus homarus) Juveniles Fed with Different Spirulina Concentration in Formulated Diet

Fawzan Bhakti Soffa, Idham Sumarto Pratama, Asep Ridwanudin, Varian Fahmi

Abstract


Spiny lobster Panulirus homarus is susceptible to the color alteration in captivity, and it might affect on the market acceptance by the customers. On the other hand, Spirulina is known as carotenoids source that is required for pigmentation, growth performance and survival rate for spiny lobster. This study addressed the effect of Spirulina inclusion in the diet of juveniles P. homarus to minimize color alteration and improve growth performance. This study was performed in Aquaculture Laboratory of the Research and Development Division for Marine Bio Industry – Indonesian Institute of Sciences (LIPI), North Lombok, West Nusa Tenggara, Indonesia. There were three replicates of three formulated feeds (0%, 4% and 8% Spirulina inclusion) and one replicate of natural feed (minced trash fish, TF). Juveniles were individually weighed and photographed to determine growth performance and color alteration in 6 body parts on carapace and abdomen using HSV (hue, saturation, value) color quantification method. After 97 days rearing period, compared to the initial color, changing body color were detected in all body parts of P. homarus and made them look brighter. Moreover, the inclusion of Spirulina in the formulated diet did not affect on specific growth rate and weight gain of spiny lobster. However, the survival rate of spiny lobster in formulated diets were better than trash fish diet. In conclusion, although the inclusion of Spirulina in the formulated diet did not enhance either in pigmentation level or growth performance of spiny lobster, utilization of formulated diet in spiny lobster culture are potential to prevent alteration of body color and also the mortality of P. homarus during the rearing period.

Keywords: Spirulina, juvenile, Panulirus homarus, diet, color
Abstrak
Perubahan Warna dan Performa Pertumbuhan Juvenil Lobster Pasir (Panulirus homarus) yang diberi Pakan Buatan dengan Konsentrasi Spirulina Berbeda. Lobster pasir Panulirus homarus yang dibudidayakan rentan mengalami perubahan warna menjadi lebih pucat, sehingga dapat memengaruhi tingkat penerimaan konsumen terhadap produk tersebut di pasaran. Spirulina merupakan salah satu sumber karotenoid yang dibutuhkan lobster dalam proses pigmentasi, pertumbuhan dan kelangsungan hidup. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan Spirulina dalam pakan buatan terhadap perubahan warna tubuh dan performa pertumbuhan pada juvenile lobster pasir. Penelitian dilakukan di Laboratorium Budidaya Balai Bio Industri Laut-LIPI, Lombok Utara, Nusa Tenggara Barat. Perlakuan penambahan tepung Spirulina dengan konsentrasi berbeda (0%, 4% dan 8%) pada pakan buatan dilakukan sebanyak tiga ulangan, sedangkan perlakuan pakan rucah sebagai kontrol dilakukan tanpa ulangan. Setiap biota uji ditimbang dan difoto untuk dapat diamati tingkat pertumbuhannya serta perubahan warna yang terjadi di enam bagian tubuhnya menggunakan metode kuantifikasi warna HSV (hue, saturation, value). Setelah 97 hari pemeliharaan, warna seluruh bagian tubuh lobster mengalami perubahan menjadi lebih cerah dibandingkan warna awal juvenil. Jika dibandingkan dengan perlakuan ikan rucah, perlakuan pakan dengan tambahan Spirulina berhasil meningkatkan rasio kelulushidupan namun tidak memengaruhi laju pertumbuhan spesifik serta pertambahan berat tubuh biota uji. Meskipun penambahan Spirulina tidak memberikan pengaruh yang signifikan terhadap pigmentasi dan pertumbuhan, penggunaan pakan buatan dalam budidaya lobster pasir memiliki potensi yang besar dalam mencegah perubahan warna dan mengurangi tingkat kematian selama pemeliharaan. Kata kunci: Spirulina, juvenil, Panulirus homarus, pakan, warna
 




Keywords


Spirulina, juvenile, Panulirus homarus, diet, color

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References


Aakermann T, OM Skulberg & S Liaaen-Jensen. 1992. A comparison of the carotenoids of strains of Oscillatoria and Spirulina (Cyanobacteria). Biochmeical Systematics and Ecology, 20(8): 761-769.

ACIAR. 2009. Final report: Assessing the South-East Asian tropical lobster supply and major market demands. Australian Center for International Agricultural Research, Canberra. p. 33.

Ang T. 2012. Digital photographer’s handbook. Fully updated 5th edition. DK Publishing. New York. 408 pp.

Astorg P. 1997. Food carotenoids and cancer prevention: An overview of current research. Trends Food Science Technology 18: 406—413.

Barclay MC, SJ Irvin, KC Williams & DM Smith. 2006. Comparison of diets for the tropical spiny lobster Panulirus ornatus: Astaxanthin-supplemented feeds and mussel flesh. Aquaculture Nutrition 12: 117—125.

Baykan NA, N Yilmaz & G Kansun. 2010. Case study in effects of color spaces for mineral identification. Scientific Research and Essays 5(11): 1243—1253.

Belay A, T Kato & Y Ota. 1996. Spirulina (Arthrospira): Potential application as an animal feed supplement. Journal of Applied Phycology 8: 303—311.

Boonyaratpalin M, S Thongrod, K Supamattaya, G Britton & LE Schlipalius. 2001. Effects of β-carotene source, Dunaliella salina, and astaxanthin on pigmentation, growth, survival and health of Penaeus monodon. Aquaculture Research, 32, 182-190.

Camgöz N, C Yener & D Güvenç. 2002. Effects of hue, saturation, and brightness on preference. Color Research and Application 27(3): 199—207.

Cantrell K, MM Erenas, I de Orbe-Paya & LF Capitan-Vallvey. 2010. Use of the hue parameter of the hue, saturation, value color space as a quantitative analytical parameter for bitonal optical sensors. Analytical Chemistry 82: 531—542.

Cattin P. 2016. Digital image fundamentals: Introduction to signal and image processing. https://miac.unibas.ch/SIP/02-Fundamentals.html#abstract. 1 April 2019.

Chen C & W Wu. 2005. Color pattern recognition with the multi-channel non-zero-order joint transform correlator based on the HSV color space. Optics Communications 244: 51–59.

Chien Y & W Shiau. 2005. The effects of dietary supplementation of algae and synthetic astaxanthin on body astaxanthin, survival, growth, and low dissolved oxygen stress resistance of kuruma prawn, Marsupenaeus japonicus Bate. Journal of Experimental Marine Biology and Ecology 318(2): 201—211.

Cianci M, PJ Rizkallah, A Olczak, J Raftery, NE Chayen, PF Zagalsky & JR Helliwell. 2002. The molecular basis of the coloration mechanism in lobster shell: β-Crustacyanin at 3.2-Å resolution. Proceedings of the National Academy of Sciences of the United States of America (PNAS). 99(15). 9795-9800.

Cuzon G, RD Santos, M Hew & G Poullaouec. 1981. Use of Spirulina in shrimp (Penaeus japonicas) diet. J. World Maricul. Soc. 12(2): 282-291.

D’abramo LR, NA Baum, CE Bordner & DE Conklin. 1983. Carotenoids as a source of pigmentation in juvenile lobsters fed a purified diet. Canadian Journal of Fisheries and Aquatic Sciences 40: 699—704.

Diaz-Jimenez J, MP Hernandez-Vergara & CI Perez-Rostro. 2018. The effect of background colour and lighting of the aquarium on the body pigmentation of the peppered shrimp Lysmata wurdemanni. Aquaculture Research. 49:3508–3516.

Duarte RC, AAV Flores, C Vinagre & MC Leal. 2017. Habitat-dependent niche partitioning between colour morphs of the algal-dwelling shrimp Hippolyte obliquimanus. Marine Biology 164: 215.

Ghaeni M, L Roomiani & Y Moradi. 2015. Evaluation of carotenoids and chlorophyll as natural resources for food in Spirulina microalgae. Applied Food Biotechnology, 2(1), 39-44.

Gokay MK & IB Gundogdu. 2008. Color identification of some Turkish marbles. Construction and Building Materials 22: 1342–1349.

Goodwin TW. 1984. The biochemistry of the carotenoids, volume II Animals. 2nd edition. Chapman and Hall, London. 213 pp.

Gora A, V Jayasankar, S Rehman, JK Kizhakudan, P Laxmilatha & P Vijayagopal. 2018. Biochemical responses of juvenile rock spiny lobster Panulirus homarus under different feeding regimes. Journal of Applied Animal Research, 46(1). 1461-1468.

Johnston D, R Melville-Smith, B Hendriks & B Phillips. 2008. Growth rates and survival of western rock lobster (Panulirus cygnus) at two temperatures (ambient and 23 ºC) and two feeding frequencies. Aquaculture 279: 77—84.

Kim CJ, SK Yoon, HI Kim, YH Park & HM Oh. 2006. Effect of Spirulina platensis and probiotics as feed additives on growth of shrimp Fenneropeaeus chinensis. J. Microbiol. Biotechnol. 16(8): 1248-1254.

Liao W, SA Nur-E-Bohran, S Okada, T Matsui & K Yamaguchi. 1993. Pigmentation of cultured black tiger prawn by feeding with a Spirulina-supplemented diet. Nippon Suisan Gakkaishi 59(1): 165—169.

Marchese G, QP Fitzgibbon, AJ Trotter, CG Carter, CM Jones & GG Smith. 2019. The influence of flesh ingredients format and krill meal in growth and feeding behavior of juvenile tropical spiny lobster Panulirus ornatus. Aquaculture 499: 128—139.

Melville-Smith R, Cheng YW & Thomson AW. 2003. Factors affecting colour change in ‘white’ western rock lobsters, Panulirus cygnus. Journal of Experimental Marine Biology and Ecology, 291: 111-129.

Menasveta P, W Worawattanamateekul, T Latscha & JS Clark. 1993. Correction of black tiger prawn (Penaeus monodon Fabricius) coloration by astaxanthin. Aquacultural Engineering 12: 203—213.

Milani A, M Basirnejad, S Shahbazi & A Bolhassani. 2017. Carotenoids: Biochemistry, pharmacology and treatment. British Journal of Pharmacology 174: 1290—1324.

Pakravan S, A Akbarzadeh, MM Sajjadi, A Hajimoradloo & F Noori. 2017. Partial and total replacement of fish meal by marine microalga Spirulina platensis in the diet of Pacific white shrimp Litopenaeus vannamei: Growth, digestive enzyme activities, fatty acid composition and responses to ammonia and hypoxia stress. Aquaculture Research 48: 5576—5586.

Pan C, Y Chien & J Cheng. 2001. Effects of light regime, algae in the water, and dietary astaxanthin on pigmentation, growth, and survival of black tiger prawn Penaeus monodon post-larvae. Zoological Studies 40(4): 371—382.

Parisenti J, LH Beirão, JL Mouriňo, FN Vieira, CC Buglione & M Maraschim. 2011. Effect of background color on shrimp pigmentation. Boletim do Instituto de Pesca, 37(2):177-182.

Park WS, H Kim, M Li, DH Lim, J Kim, S Kwak, C Kang, MG Feruzzi & M Ahn. 2018. Two classes of pigments, carotenoids and c-phycocyanin, in Spirulina powder and their antioxidant activities. Molecules, 23, 2065-2075.

Rao KR. 2001. Crustacean pigmentary-effector hormones: Chemistry and functions of RPCH, PDH, and related peptides. American Zoologist 41: 364—379.

Russel BJ & HM Dierssen. 2015. Use of hyperspectral imagery to assess cryptic color matching in Sargassum associated crabs. PLOS ONE | DOI:10.1371/journal.pone.0136260.

Smith DM, KC Williams & SJ Irvin. 2005. Response of the tropical spiny lobster Panulirus ornatus to protein content of pelleted feed and to a diet of mussel flesh. Aquaculture Nutrition 11: 209—217.

Tlusty MF. 2005. Use of digital colour analysis to assess variation within individual adult American lobsters (Homarus americanus) and the process of addition of colour in white lobsters. New Zealand Journal of Marine and Freshwater Research 39: 571—580.

Tlusty MF, JS Goldstein & DR Fiore. 2005. Hatchery performance of early benthic juvenile American lobsters (Homarus americanus) fed enriched frozen adult Artemia diets. Aquaculture Nutrition 11: 191—198.

Tlusty MF, A Myers & A Metzler. 2008. Short- and long-term dietary effects on disease and mortality in American lobster Homarus americanus. Diseases of Aquatic Organisms, 78, 249-253.

Tohda K & M Gratzl. 2006. Micro-miniature autonomous optical sensor array for monitoring ions and metabolites 1: Design, fabrication, and data analysis. Analytical Sciences 22: 383—388.

Tseng K, H Su & M Su. 1998. Culture of Penaeus monodon in a recirculating system. Aquacultural Engineering, 17, 138-147.

Tume RK, AL Sikes, S Tabrett & DM Smith. 2009. Effect of background colour on the distribution of astaxanthin in black tiger prawn (Penaeus monodon): Effective method for improvement of cooked colour. Aquaculture, 296, 129-135.

Wade NM, R Melville-Smith, BM Degnan & MR Hall. 2008. Control of shell colour changes in the lobster, Panulirus cygnus. The Journal of Experimental Biology, 211, 1512-1519.

Wade NM, M. Anderson, MJ Sellars, RK Tume, NP Preston & BD Glencross. 2012. Mechanisms of colour adaptation in the prawn Penaeus monodon. Journal of Experimental Biology 215: 343—350.

Wade NM, J Gabaudan & BD Glencross. 2017. A review of carotenoid utilisation and function in crustacean aquaculture. Reviews in Aquaculture, 9, 141-156.

Wang Y, Y Liu, W Liu, W Tang, L Shen, Z Li & M. Fan. 2018. Quantification of combined color and shade changes in colorimetry and image analysis: Water pH measurement as an example. Analytical Methods. The Royal Society of Chemistry 10: 3059—3065.

Yam KL & SE Papadakis. 2004. A simple digital imaging method for measuring and analyzing color of food surfaces. Journal of Food Engineering 61(1): 137—142.

You K, H Yang, Y Liu, S Liu, Y Zhou & T Zhang. 2006. Effects of different light sources and illumination methods on growth and body color of shrimp Litopenaeus vannamei. Aquaculture, 252, 557-565.


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